Indonesia needs asphalt mixture with good quality and high resistance against daily heavy traffic.  One of the ways is to modified asphalt mixture with High Density Polyethylene (HDPE). This is also useful for reducing environmental problems due to daily use of plastic. This study investigates the performance of asphalt mixture with HDPE substitution with variants of 0%, 2%, 4% and 6% by asphalt weight in the mixture. In this study, the asphalt mixing process conducted by two methods such as wet and dry method and using the original seeds of HDPE and chopped HDPE. The performance of asphalt mixture with HDPE substitution was analyzed using Marshall test that based on Asphalt Pavement Spesification (Bina Marga, 2018) and Cantabro Abrasion Loss (CAL) test in terms of strength, stiffness, and durability characteristics. Laboratory result showed that asphalt mixture substitution by original seeds of HDPE with dry method gives the maximum stability that is 1970.8 kg at 6% HDPE content. But, only in level content of 2-4% HDPE substitution which qualifying the specification such as Stability, VIM, VMA, VFA, MQ (Marshall parameters).